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bj fibroblast foreskin ectoderm atcc  (ATCC)


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    ATCC bj fibroblast foreskin ectoderm atcc
    Bj Fibroblast Foreskin Ectoderm Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bj+foreskin+fibroblast/BJ/us12653888-350-19-23
    Average 99 stars, based on 1775 article reviews
    bj fibroblast foreskin ectoderm atcc - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Efficient RNA-mediated reprogramming of human somatic cells to naïve pluripotency facilitated by tankyrase inhibition
    Article Snippet: .. Adult human dermal fibroblasts (HDFa), HDFa16, HDFa75 (Thermo Fisher Scientific C0135C), and BJ foreskin fibroblast (ATCC® CRL-2522TM) were cultured in DMEM high glucose (Sigma D5546) with FBS (10%, Sigma, F0804), L-glutamine (2mM, Invitrogen, 25030-024) and 2-mercaptoethanol (100μM, Sigma, M3148) on gelatin-coated plates. .. Peripheral blood-derived endothelial progenitor cells (EPC, C26b) were cultured as described ( ) in endothelial cell basal medium (PromoCell, c-22210) supplemented with 10% FBS and cytokines without heparin.

    Article Title: Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency
    Article Snippet: .. Adult HDFs (HDFa), HDFa16, HDFa75 (Thermo Fisher Scientific, C0135C), and BJ foreskin fibroblast (ATCC, CRL-2522) were cultured in DMEM high glucose (Merck, D5546) with FBS (10%, Merck, F0804), L-glutamine (2 mM, Thermo Fisher Scientific, 25030024) and 2-mercaptoethanol (100 μM, Merck, M3148) on 0.1% gelatin-coated plates. .. Peripheral blood-derived EPCs (C26b, EPC1, and EPC2) were cultured as described ( ) in endothelial cell basal medium (PromoCell, c-22210) supplemented with 10% FBS and cytokines, without heparin.

    Retroviral:

    Article Title: Replication Kinetics for a Reporter Merkel Cell Polyomavirus
    Article Snippet: .. BJ-hTert cells were established by retroviral transduction of Babe hTert-puro (pBabe-hTert-puro: a gift from Roderick J. O’Sullivan, Hillman Cancer Center, University of Pittsburgh, Pittsburgh, PA, USA) into primary BJ foreskin fibroblast (ATCC CRL-2522) and selected with 2 μg/mL puromycin. .. To establish 293 TRE-sTco cells, 293 (ATCC CLR-1573) were transduced with pLenti TRE MCV sT [ ] and selected with 2 μg/mL puromycin.

    Transduction:

    Article Title: Replication Kinetics for a Reporter Merkel Cell Polyomavirus
    Article Snippet: .. BJ-hTert cells were established by retroviral transduction of Babe hTert-puro (pBabe-hTert-puro: a gift from Roderick J. O’Sullivan, Hillman Cancer Center, University of Pittsburgh, Pittsburgh, PA, USA) into primary BJ foreskin fibroblast (ATCC CRL-2522) and selected with 2 μg/mL puromycin. .. To establish 293 TRE-sTco cells, 293 (ATCC CLR-1573) were transduced with pLenti TRE MCV sT [ ] and selected with 2 μg/mL puromycin.



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    Image Search Results


    (A) BF images five out of the seven cell lines, including telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.

    Journal: bioRxiv

    Article Title: Epigenetic–splicing regulation of hTERT mediated by hTAPAS

    doi: 10.64898/2026.05.08.723733

    Figure Lengend Snippet: (A) BF images five out of the seven cell lines, including telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.

    Article Snippet: Human embryonic kidney 293T (HEK293T; ATCC® CRL-3216TM), human embryonic lung fibroblast VA13 (WI-38 VA13 subline 2RA; ATCC® CCL-75.1TM), human foreskin fibroblast HFF-1 (ATCC® SCRC-1041TM), human lung fibroblast IMR-90 (ATCC® CCL-186TM), and human foreskin fibroblast BJ (ATCC® CRL-2522TM) cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Expressing

    Targeted DNA methylation profiling was performed using CRISPR–Cas9 enrichment followed by Nanopore sequencing across a ∼9 kb region spanning hTAPAS through hTERT intron 2 (Chr. 5: 1,196,006–1,205,206) and a ∼6.5 kb region covering introns 6–8 (Chr. 5: 1,174,035–1,180,535). The analysis included telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive cell lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive cell line VA13. DNA methylation levels at individual CpG sites are depicted across the indicated genomic regions, including hTAPAS , the THOR region, the core promoter, exon1, intron1, exon 2 and intron 2. Methylation for each individual CpG is shown as a percentage, with unmethylated CpGs depicted in red and methylated CpGs in blue. Methylation across intron 2 was consistently high (80–100%) in all cell lines, whereas regions encompassing hTAPAS , the THOR region, exon 2, and introns 6–8 displayed marked variability between cell types. CpGs within the hTAPAS region were highly methylated in telomerase-positive cells and in the ALT-positive VA13 line, but largely unmethylated in fibroblasts, with partial methylation observed in IMR90. The core hTERT promoter and exon 2–proximal regions remained mostly unmethylated in all cell lines except VA13, which exhibited substantial hypermethylation

    Journal: bioRxiv

    Article Title: Epigenetic–splicing regulation of hTERT mediated by hTAPAS

    doi: 10.64898/2026.05.08.723733

    Figure Lengend Snippet: Targeted DNA methylation profiling was performed using CRISPR–Cas9 enrichment followed by Nanopore sequencing across a ∼9 kb region spanning hTAPAS through hTERT intron 2 (Chr. 5: 1,196,006–1,205,206) and a ∼6.5 kb region covering introns 6–8 (Chr. 5: 1,174,035–1,180,535). The analysis included telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive cell lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive cell line VA13. DNA methylation levels at individual CpG sites are depicted across the indicated genomic regions, including hTAPAS , the THOR region, the core promoter, exon1, intron1, exon 2 and intron 2. Methylation for each individual CpG is shown as a percentage, with unmethylated CpGs depicted in red and methylated CpGs in blue. Methylation across intron 2 was consistently high (80–100%) in all cell lines, whereas regions encompassing hTAPAS , the THOR region, exon 2, and introns 6–8 displayed marked variability between cell types. CpGs within the hTAPAS region were highly methylated in telomerase-positive cells and in the ALT-positive VA13 line, but largely unmethylated in fibroblasts, with partial methylation observed in IMR90. The core hTERT promoter and exon 2–proximal regions remained mostly unmethylated in all cell lines except VA13, which exhibited substantial hypermethylation

    Article Snippet: Human embryonic kidney 293T (HEK293T; ATCC® CRL-3216TM), human embryonic lung fibroblast VA13 (WI-38 VA13 subline 2RA; ATCC® CCL-75.1TM), human foreskin fibroblast HFF-1 (ATCC® SCRC-1041TM), human lung fibroblast IMR-90 (ATCC® CCL-186TM), and human foreskin fibroblast BJ (ATCC® CRL-2522TM) cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: DNA Methylation Assay, CRISPR, Nanopore Sequencing, Methylation